Methods

We use modern and highly efficient methods for our various molecular biological experiments. Our vectors are mainly based on the MoClo system, which is why, in addition to PCR-based cloning methods (EMP-PCR) and Gibson assembly, cut ligation is usually our cloning method of choice. The transformation of plants is performed with all commonly available protocols like Agrobacteria-mediated or ballistic transformation as well as electroporation. In Wolffia we also use protoplasts, which we can produce in large quantities.

Finally, genome editing using CRISPR/Cas9 has also been performed in our group.