Transcriptional reprogramming

Transcriptional reprogramming in arbuscular mycorrhizal roots

Based on cell- and development-specific gene expression profiles, we try to identify key factors mediating the pre-symbiotic communication as well as the subsequent infection and colonization of Medicago truncatula roots by arbuscular mycorrhizal fungi of the order Glomeromycota.

Functional analyses of selected transcriptional regulators and other candidates for symbiont recognition and function are performed in transgenic knock-down roots or mutant lines, via protein-protein interaction assays, and by subcellular localization studies using confocal laser scanning microscopy.

By combining molecular genetic, histological, microscopic, and physiological approaches, insights into key molecular switches governing important early and late reprogramming steps during the successful establishment of an arbuscular mycorrhizal symbiosis are obtained.